Microsatellite DNA Loci Reveal Successful Cross-Genus Amplification in a Critically Endangered Parashorea malaanonan

Randy A. Villarin

doi:10.61569/bdj1dt93

Microsatellite DNA Loci Reveal Successful Cross-Genus Amplification in a Critically Endangered Parashorea malaanonan

Authors

Randy A. Villarin Naval State University Author

DOI:

https://doi.org/10.61569/bdj1dt93

Keywords:

Cross-amplification, Microsatellites/SSRs, Parashorea malaanonan, Dipterocarpaceae

Abstract

Parashorea malaanonan, of the family Dipterocarpaceae, is one of the most economically and ecologically important timber species in the Philippines. Presently, genetic resource of this species is highly threatened and its long-term survival is critically endangered. Hence, genetic investigation for the species is very essential. In this study, 23 microsatellite DNA loci developed for Shorea leprosula and S. curtisii were cross-amplified to P. malaanonan. This results a high transferability rate of 74%. The findings provided additional evidence for the potential cross-amplification of Shorea SSRs across genera within Dipterocarpaceae. The five polymorphic microsatellites are useful for further genetic studies of the remaining P. malaanonan populations.

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Published

2015-12-10

Issue

Vol. 3 No. 1 (2015): Journal of Science, Engineering and Technology

Section

Articles

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the license is given, and indication of whether changes were made. See: Creative Commons Attributions 4.0 International License.